Neutrophil Gelatinase Associated Lipocalin / NGAL (LCN2) Antibody

NGAL/LCN2 Antibody is a Rabbit Polyclonal antibody against NGAL. Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis.

Target	Neutrophil Gelatinase Associated Lipocalin / NGAL (LCN2)
Clonality	Polyclonal
Reactivity	Human, Mouse, Rat
Tested Applications	WB, IF/ICC
Host	Rabbit
Recommended dilutions	WB: 1/500 - 1/1000, IF/ICC: 1/50 - 1/200. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Lipocalin-2/NGAL.
Isotype	IgG
Form	Liquid
Purification	Purified by affinity chromatography.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	P80188 (UniProt, ExPASy)
UniProt Entry Name	NGAL_HUMAN
Gene Symbol	LCN2
GeneID	3934
OMIM	600181
NCBI Accession	NP_005555.2
HGNC	6526
KEGG	hsa:3934
Ensembl	ENSG00000148346
String	9606.ENSP00000362108
Molecular Weight	Calculated MW: 23 kDa Observed MW: 22 kDa
Buffer	PBS, pH 7.3, containing 0.01% thimerosal, 50% glycerol.
Concentration	> 0.2 mg/ml
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on Neutrophil Gelatinase Associated Lipocalin / NGAL (LCN2)

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