Lymphoid Enhancer Binding Factor 1 (LEF1) Antibody

LEF1 Antibody is a Rabbit Polyclonal antibody against LEF1. Lymphoid enhancer-binding factor 1(LEF1) belongs to a family of regulatory protein share homology with high mobility group protein-1, and it's a nuclear protein exprssed in pre-B and T cells. LEF1 has a role in the Wnt signaling pathway and hair cell differentiation and follicle morphogenesis. Together with CTNNB1 and EP300, LEF1 activates transcription of target genes. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells.

Target	Lymphoid Enhancer Binding Factor 1 (LEF1)
Clonality	Polyclonal
Reactivity	Human, Mouse, Rat
Tested Applications	WB, IF/ICC, IP
Host	Rabbit
Recommended dilutions	WB: 1/500 - 1/1000, IP: 0.5-4 µg/200-400 µg whole cell extracts, IF/ICC: 1/50 - 1/200. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 20-132 of human LEF1.
Isotype	IgG
Form	Liquid
Purification	Purified by affinity chromatography.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	Q9UJU2 (UniProt, ExPASy)
UniProt Secondary AC	B4DG38, B7Z8E2, E9PDK3, Q3ZCU4, Q9HAZ0
UniProt Entry Name	LEF1_HUMAN
Gene Symbol	LEF1
GeneID	51176
NCBI Accession	NP_057353.1
String	9606.ENSP00000265165
Molecular Weight	Calculated MW: 44 kDa Observed MW: 25-58 kDa
Buffer	PBS, pH 7.3, containing 0.05% Proclin-300, 50% glycerol.
Concentration	> 0.2 mg/ml
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on Lymphoid Enhancer Binding Factor 1 (LEF1)

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