Human Lemur Tyrosine Kinase 3 (LMTK3) Protein (Active)
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Catalogue No: abx651405
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(Size: 50 µg)
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Datasheet SDS
Human Lemur Tyrosine Kinase 3 (LMTK3) Protein (Active) is an active Human protein expressed in E. coli.
Research Articles on Lemur Tyrosine Kinase 3 (LMTK3)
| Target | Lemur Tyrosine Kinase 3 (LMTK3) |
| Research Area | Enzymes and Kinases |
| Origin | Human |
| Expression | Recombinant |
| Tested Applications | WB, SDS-PAGE |
| Host | E. coli |
| Conjugation | Unconjugated |
| Form | Lyophilized |
| Activity | Active |
| Biological Activity | Lemur tyrosine kinase 3 (LMTK3) is implicated in breast cancer growth and endocrine resistance, and has been reported to promote cell invasion, motility, and migration of the MCF-7 breast cancer cell line. A proliferation assay was conducted to detect the bioactivity of recombinant Human LMTK3 using MCF-7 cells. Briefly, MCF-7 cells were seeded into triplicate wells in 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free DMEM prior to the addition of various concentrations of LMTK3. After incubation for 72 h, cells were observed by inverted microscope (Figures 3 and 4) and cell proliferation was measured by Cell Counting Kit-8 (CCK-8) assay (Figure 5). Briefly, 10 µl of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 °C. |
| Purity | > 95% |
| Reconstitution | Reconstitute in ddH2O to a concentration up to 0.2 mg/ml. Do not vortex. |
| Storage | Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze–thaw cycles. |
| Shelf Life | 12 months. |
| UniProt Primary AC | Q96Q04 (UniProt, ExPASy) |
| UniProt Secondary AC | Q4G0U1 |
| UniProt Entry Name | LMTK3_HUMAN |
| Gene Symbol | LMTK3 |
| GeneID | 114783 |
| HGNC | 19295 |
| String | 9606.ENSP00000270238 |
| Molecular Weight | Calculated MW: 45.9 kDa Observed MW (SDS-PAGE): 46 kDa |
| Sequence Fragment | Leu133-Leu411 |
| Sequence | LSYLQEIG SGWFGKVILG EIFSDYTPAQ VVVKELRASA GPLEQRKFIS EAQPYRSLQH PNVLQCLGLC VETLPFLLIM EFCQLGDLKR YLRAQRPPEG LSPELPPRDL RTLQPMGLEI ARGLAHLHSH NYVHSDLALR NCLLTSDLTV RIGDYGLAHS NYKEDYYLTP ERLWIPLRWA APELLGELHG TFMVVDQSRE SNIWSLGVTL WELFEFGAQP YRHLSDEEVL AFVVRQQHVK LARPRLKLPY ADYRYDILQS CWRPPAQRPS ASDLQLQLTY L |
| Tag | N-terminal His tag and SUMO tag |
| Buffer | Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 0.01% Sarcosyl, 5% Trehalose. |
| Endotoxin Level | < 1.0 EU/µg (LAL method) |
| Concentration | Prior to lyophilization: 200 µg/ml |
| Availability | Shipped within 5-7 working days. |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. |