Human Leptin (LEP) Protein (Active)
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Catalogue No: abx651406
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US$348.00
(Size: 50 µg)
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Datasheet SDS
Human Leptin (LEP) Protein (Active) is an active protein from Human. This protein exhibits binding activity as detailed in the Biological Activity section below. Other types of biological activity, such as enzymatic activity, have not been tested.
Research Articles on Leptin (LEP)
| Target | Leptin (LEP) |
| Research Area | Metabolic Pathways, Endocrinology, Cardiovascular Biology |
| Origin | Human |
| Expression | Recombinant |
| Tested Applications | WB, SDS-PAGE |
| Host | E. coli |
| Conjugation | Unconjugated |
| Form | Lyophilized |
| Activity | Active |
| Biological Activity | A binding ELISA assay was conducted to detect the interaction of recombinant Human LEP and recombinant human HK3. Briefly, LEP was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to HK3-coated microplate wells and incubated for 2 h at 37 °C. Wells were washed with PBST and incubated for 1 h with anti-LEPpAb, then aspirated and washed 3 times. After incubation with HRP-conjugated secondary antibody, wells were aspirated and washed 3 times. TMB substrate solution was added and wells were incubated for 15-25 minutes at 37 °C. Finally, 50 μl stop solution was added to the wells and the absorbance was read at 450 nm immediately. The binding activity of of LEP and HK3 is shown in the figures. A dose-dependent effect was observed. |
| Purity | > 90% |
| Reconstitution | To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0. If a higher concentration is required, the product can be reconstituted directly in 20 mM Tris, 150 mM NaCl, pH 8.0, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex. |
| Storage | Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze–thaw cycles. |
| Shelf Life | 12 months. |
| UniProt Primary AC | P41159 (UniProt, ExPASy) |
| UniProt Secondary AC | O15158, Q56A88 |
| UniProt Entry Name | LEP_HUMAN |
| KEGG | hsa:3952 |
| String | 9606.ENSP00000312652 |
| Molecular Weight | Calculated MW: 46.0 kDa Observed MW: 46 kDa |
| Sequence Fragment | Val22-Cys167 |
| Tag | N-terminal His tag and GST tag |
| Buffer | Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01% Sarcosyl, 5% Trehalose and Proclin-300. |
| Endotoxin Level | < 1.0 EU/µg protein (LAL method) |
| Concentration | Prior to lyophilization: 200 µg/ml |
| Availability | Shipped within 5-7 working days. |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. |