DNA Ligase 4 (LIG4) Antibody

LIG4 Antibody is a Rabbit Polyclonal antibody against LIG4. Two major pathways, homologous recombination (HR) and nonhomologous end joining (NHEJ), counteract one of themost toxic lesions, the DSB. The core protein complex mediating NHEJ in mammals includes DNA ligase IV (Lig4). Lig4 belongs to an ATP-dependent DNA ligase family, and joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. The complex Lig4-XRCC4 is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of Lig4.

Target	DNA Ligase 4 (LIG4)
Clonality	Polyclonal
Reactivity	Human, Mouse, Rat
Tested Applications	WB, IHC
Host	Rabbit
Recommended dilutions	WB: 1/500 - 1/1000, IHC-P: 1/50 - 1/200. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human DNA Ligase IV.
Isotype	IgG
Form	Liquid
Purification	Purified by affinity chromatography.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	P49917 (UniProt, ExPASy)
UniProt Secondary AC	Q8IY66, Q8TEU5
UniProt Entry Name	DNLI4_HUMAN
Gene Symbol	LIG4
GeneID	3981
NCBI Accession	NP_002303.2
KEGG	hsa:3981
String	9606.ENSP00000484288
Molecular Weight	Calculated MW: 104 kDa Observed MW: 110 kDa
Buffer	PBS, pH 7.3, containing 0.09% sodium azide, 50% glycerol.
Concentration	> 0.2 mg/ml
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on DNA Ligase 4 (LIG4)

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