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Luciferase Enzyme (Active)
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Catalogue No: abx074305
Price:
US$261.00
(Size: 1 µg)
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Firefly Luciferase is a recombinant enzyme expressed in E. coli.
Research Articles on Luciferase
| Target | Luciferase |
| Origin | Insect |
| Expression | Recombinant |
| Tested Applications | SDS-PAGE |
| Host | E. coli |
| Recommended dilutions | Optimal dilutions/concentrations should be determined by the end user. |
| Form | Liquid |
| Purity | > 95% (SDS-PAGE) |
| Purification | Purified by proprietary chromatographic techniques. |
| Storage | Store at 4 °C if the entire vial will be used within 4 weeks. Store at -20 °C for long term storage. For long term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze/thaw cycles. |
| UniProt Primary AC | P08659 (UniProt, ExPASy) |
| UniProt Secondary AC | Q27755 |
| UniProt Entry Name | LUCI_PHOPY |
| Molecular Weight | 38.5 kDa |
| Sequence Fragment | 1-311 AA. |
| Sequence | MGSSHHHHHH SSGLVPRGSH MGSH-MTSKVY DPEQRKRMIT GPQWWARCKQ MNVLDSFINY YDSEKHAENA VIFLHGNAAS SYLWRHVVPH IEPVARCIIP DLIGMGKSGK SGNGSYRLLD HYKYLTAWFE LLNLPKKIIF VGHDWGACLA FHYSYEHQDK IKAIVHAESV VDVIESWDEW PDIEEDIALI KSEEGEKMVL ENNFFVETML PSKIMRKLEP EEFAAYLEPF KEKGEVRRPT LSWPREIPLV KGGKPDVVQI VRNYNAYLRA SDDLPKMFIE SDPGFFSNAI VEGAKKFPNT EFVKVKGLHF SQEDAPDEMG KYIKSFVERV LKNEQ |
| Tag | N-terminal His tag |
| Buffer | 20 mM Tris-HCl, pH 8.0, containing 1 mM DTT and 10% glycerol. |
| Activity | Active |
| Biological Activity | Specific activity: > 1 × 109 light units/mg One luciferase enzyme unit will produce one Relative Light Unit (RLU) at pH 7.5 at 25 °C. |
| Concentration | 0.5 mg/ml |
| Availability | Shipped within 5-10 working days. |
| Note | This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use. |
