M199 (Powder)

Medium 199 (M199) is widely used in the culture of various animal cells, including some non-mammalian cells. Compared with other media, M199 contains adenine, adenosine, hypoxanthine, thymine, and other vitamins. It contains various amino acids and vitamins essential for cell growth, though does not contain proteins, lipids or growth factors.

The 1 × 10 L size is provided as 1 bottle containing lyophilized powder for 10 L of 1X M199. The 1 × 50 L size is provided as 1 bottle containing lyophilized powder for 50 L of 1X M199.

Target	M199
Form	Lyophilized
Reconstitution	Reconstitute in distilled water.
Storage	Store between 2-8 °C. Avoid exposure to light.
Validity	Up to 36 months.
Buffer	After reconstitution: pH 7.2-7.4, containing 1000 mg/L D-glucose, 100 mg/L L-glutamine, 20 mg/L phenol red and Earle's salt. Does not contain HEPES or NaHCO3.
Concentration	1X
Availability	Shipped within 5-7 working days.
Note	This product is for research use only.
Directions for use	This product should be used with serum (or equivalent) supplementation. Use aseptic techniques to avoid contamination.

Research Articles on M199

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