MAPK8/9/10 (pT183/Y185/T221) Antibody is a Rabbit Polyclonal antibody against MAPK8 (pT183/Y185), MAPK9 (pT183/Y185) and MAPK10 (T221). Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells By similarity. Phosphorylates heat shock factor protein 4 (HSF4). /Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells. JNK2 isoforms display different binding patterns: a-1 and a-2 preferentially bind to c-Jun, whereas beta-1 and beta-2 bind to ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms. JUNB is not a substrate for JNK2 a-2, and JUND binds only weakly to it. /Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. Required for stress-induced neuronal apoptosis and the pathogenesis of glutamate excitotoxicity.
Target |
MAPK8/9/10 (pT183/Y185/T2215) |
Reactivity |
Human, Mouse, Rat |
Origin |
Human |
Host |
Rabbit |
Clonality |
Polyclonal |
Tested Applications |
WB, IF/ICC |
Recommended dilutions |
WB: 1/500 - 1/2000, IF/ICC: 1/100 - 1/200. Optimal dilutions/concentrations should be determined by the end user. |
Immunogen |
Synthetic Peptide. A phospho specific peptide corresponding to residues surrounding T183/Y185 of human MAPK8/MAPK9/MAPK10. |
Purification |
Affinity purified. |
Form |
Liquid |
Isotype |
IgG |
Conjugation |
Unconjugated |
Storage |
Aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
Molecular Weight |
Calculated MW: 35 kDa/44 kDa/48 kDa/27 kDa/52 kDa Observed MW: 46 kDa, 54 kDa |
Swiss Prot |
P45983
P45984
P53779
|
GeneID |
5599/5601/5602
|
Gene Symbol |
MAPK8, MAPK9, MAPK10 |
Concentration |
> 1 mg/ml |
Buffer |
PBS, pH 7.3, 0.02% sodium azide, 50% glycerol. |
Availability |
Shipped within 5-10 working days. |
Note |
This product is for research use only. |