O-6-Methylguanine DNA Methyltransferase (MGMT) Antibody

MGMT Antibody is a Rabbit Polyclonal antibody against MGMT. MGMT is the primary vehicle for cellular removal of alkyl lesions from the O-6 position of guanine and the O-4 position of thymine. While key to the maintenance of genomic integrity, MGMT also removes damage induced by alkylating chemotherapies, inhibiting the efficacy of cancer treatment [PMID:23065697].MGMT is the primary mechanism for the removal of alkylation damage from the O-6 position of guanine [PMID: 17482892]. The O-6 position of guanine is one of several positions in DNA bases to which alkyl groups are attached in SN1 alkylation reactions, and this repair has been well-characterized in mammalian cells and via MGMT homologs in bacteria and Archaea.[PMID: 10767620].

Target	O-6-Methylguanine DNA Methyltransferase (MGMT)
Clonality	Polyclonal
Reactivity	Human
Tested Applications	WB
Host	Rabbit
Recommended dilutions	WB: 1/500 - 1/2000. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-207 of human MGMT.
Isotype	IgG
Form	Liquid
Purification	Purified by affinity chromatography.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	P16455 (UniProt, ExPASy)
UniProt Secondary AC	Q5VY78
UniProt Entry Name	MGMT_HUMAN
Gene Symbol	MGMT
GeneID	4255
NCBI Accession	NP_002403.2
KEGG	hsa:4255
String	9606.ENSP00000302111
Molecular Weight	Calculated MW: 22 kDa Observed MW: 22 kDa
Buffer	PBS, pH 7.3, containing 0.02% sodium azide, 50% glycerol.
Concentration	> 0.2 mg/ml
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on O-6-Methylguanine DNA Methyltransferase (MGMT)

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