Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV-RT) Protein (Active)
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Catalogue No: abx073903
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US$261.00
(Size: 2500 U)
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Datasheet SDS
Moloney Leukemia Virus Reverse Trancscriptase is a recombinant enzyme expressed in E. coli.
Research Articles on Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV-RT)
| Target | Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV-RT) |
| Origin | Virus |
| Expression | Recombinant |
| Host | E. coli |
| Recommended dilutions | Optimal dilutions/concentrations should be determined by the end user. |
| Form | Liquid |
| Activity | Active |
| Biological Activity | One unit is defined as the amount of enzyme required to catalyze the incorporation of 1 nmol of deoxyribonucleotide into acid-insoluble forms in 10 minutes at 37 °C, using poly(A)-oligo(dT)12-18 as the template-primer. Standard cDNA Synthesis Conditions: 50 mM Tris-HCl (pH 8.3), 75 mM KCl, 3 mM MgCl2, 10 mM DTT, 1.0 mM each dATP, dGTP, dCTP, and dTTP; 0.2 mg random hexamer, 1-5 mg RNA, 200 units MMLV-RT. The reaction volume was 20 ml and incubation was carried out at 42 °C for 45 min. |
| Storage | Store at -20°C for long term storage or at 4°C for up to 1 month. For long term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze/thaw cycles. |
| Shelf Life | 12 months. |
| Buffer | 50 mM Tris-HCl, 0.1 M NaCl, 0.1% Triton X-100, 2 mM DTT, 0.1 mM EDTA and 50% glycerol. |
| Concentration | 200 U/µl |
| Availability | Shipped within 5-10 working days. |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. |