Mouse Collagen Triple Helix Repeat Containing Protein 1 (CTHRC1) Protein

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Catalogue No: abx620128
Price: US$2,639.00
(Size: 1 mg)

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SDS-PAGE analysis of expression tests of the target protein using coomassie blue staining. The negative control is marked Ø. Briefly, the bacterial cells were collected by centrifugation. The supernatant (medium) was lysed in a buffer containing PBS, pH 7.5. After centrifugation, the soluble supernatnant fraction was collected (native protein extraction - NPE). The insoluble fraction was collected and solubilized with a denaturing buffer (8 M Urea). After centrifugation, the supernatant was collected (denatured protein extraction - DPE). The NPE and DPE fractions were analyzed by SDS-PAGE. The target genes were transfected in HEK293 cells, 1.5 ml of culture medium and cells were collected on 6th day of post-transfection. After semi-purification, the optimal expression was observed in cultutre medium, NPE and DPE fraction.
Mouse Collagen Triple Helix Repeat Containing Protein 1 (CTHRC1) Protein Mouse Collagen Triple Helix Repeat Containing Protein 1 (CTHRC1) Protein

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Datasheet SDS
Mouse Collagen Triple Helix Repeat Containing Protein 1 (CTHRC1) Protein is a recombinant Mouse Protein expressed in mammalian cells, containing a C-terminal His-tag.

Target Collagen Triple Helix Repeat Containing Protein 1 (CTHRC1)
Origin Mouse
Expression Recombinant
Tested Applications SDS-PAGE
Host Mammalian cells
Form Lyophilized
Activity Not tested
Storage Aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles.
Shelf Life 12 months.
UniProt Primary AC Q9D1D6 (UniProt, ExPASy)
UniProt Secondary AC Q3UAP6
UniProt Entry Name CTHR1_MOUSE
Gene Symbol CTHRC1
GeneID 68588
KEGG mmu:68588
Ensembl ENSMUSG00000054196
String 10090.ENSMUSP00000070018
Sequence Fragment 33-245 AA (Full length without the signal peptide)
Sequence SENPKVKQ KALIRQREVV DLYNGMCLQG PAGVPGRDGS PGANGIPGTP GIPGRDGFKG EKGECLRESF EESWTPNYKQ CSWSSLNYGI DLGKIAECTF TKMRSNSALR VLFSGSLRLK CRNACCQRWY FTFNGAECSG PLPIEAIIYL DQGSPELNST INIHRTSSVE GLCEGIGAGL VDVAIWVGTC SDYPKGDAST GWNSVSRIII EELPK GSHHHHHH
Tag C-terminal His tag
Buffer Prior to lyophilization: PBS, pH 7.5.
Endotoxin Level < 10 EU/mg
Concentration Prior to lyophilization: 0.60 mg/ml
Availability Shipped within 1-2 months.
Note THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
Research Articles on Collagen Triple Helix Repeat Containing Protein 1 (CTHRC1)


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