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Mouse Fatty Acid Binding Protein 4, Adipocyte (FABP4) Protein (Active)
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Catalogue No: abx655619
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US$623.50
(Size: 50 µg)
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Datasheet SDS
Mouse Fatty Acid Binding Protein 4, Adipocyte (FABP4) Protein (Active) is an Active recombinant Mouse protein. This protein exhibits binding activity as detailed in the Biological Activity section below. Other types of biological activity, such as enzymatic activity, have not been tested.
Research Articles on Fatty Acid Binding Protein 4, Adipocyte (FABP4)
| Target | Fatty Acid Binding Protein 4, Adipocyte (FABP4) |
| Research Area | Metabolism, Endocrinology, Cardiovascular Biology, Hepatology |
| Origin | Mouse |
| Expression | Recombinant |
| Tested Applications | WB, SDS-PAGE |
| Host | 293F cells |
| Conjugation | Unconjugated |
| Form | Lyophilized |
| Activity | Active |
| Biological Activity | A binding ELISA assay was conducted to detect the interaction of recombinant Mouse FABP4 and recombinant human PPARg. Briefly, FABP4 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to PPARg-coated microplate wells and incubated for 1 h at 37 °C. Wells were aspirated and incubated for 1 h with anti-FABP4 monoclonal antibody, then aspirated and washed 3 times. After incubation with HRP-conjugated secondary antibody, wells were aspirated and washed 5 times. TMB substrate solution was added and wells were incubated for 15-25 minutes at 37 °C. Finally, 50 μl stop solution was added to the wells and the absorbance was read at 450 nm immediately. The binding activity of recombinant mouse FABP4 and recombinant human PPARg is shown in the figures. A dose-dependent effect was observed. The EC50 for this effect is 36.6 µg/ml. |
| Purity | > 90% |
| Reconstitution | To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0. If a higher concentration is required, the product can be reconstituted directly in 20 mM Tris, 150 mM NaCl, pH 8.0, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex. |
| Storage | Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze–thaw cycles. |
| Shelf Life | 12 months. |
| UniProt Primary AC | P04117 (UniProt, ExPASy) |
| UniProt Entry Name | FABP4_MOUSE |
| KEGG | mmu:11770 |
| String | 10090.ENSMUSP00000029041 |
| Sequence Fragment | Please enquire. |
| Tag | Please enquire. |
| Buffer | Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01% Sarcosyl, 5% Trehalose and Proclin-300. |
| Endotoxin Level | < 1.0 EU/µg protein (LAL method) |
| Concentration | Prior to lyophilization: 200 µg/ml |
| Availability | Shipped within 5-7 working days. |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. |