Mouse Fibronectin (FN1) Protein (Active)

Mouse Fibronectin (FN1) is an active recombinant Mouse protein produced in a Prokaryotic expression system (E. coli).

Target	Fibronectin (FN1)
Research Area	Signal Transduction, Infection Immunity
Origin	Mouse
Expression	Recombinant
Tested Applications	WB, SDS-PAGE
Host	E. coli
Conjugation	Unconjugated
Form	Lyophilized
Purity	> 98%
Reconstitution	To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH₂O. If a lower concentration is required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0. If a higher concentration is required, the product can be reconstituted directly in 20 mM Tris, 150 mM NaCl, pH 8.0, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
Storage	Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	P11276 (UniProt, ExPASy)
UniProt Secondary AC	G5E8B8, Q61567, Q61568, Q61569, Q64233, Q80UI4
UniProt Entry Name	FINC_MOUSE
Gene Symbol	FN1
GeneID	14268
KEGG	mmu:14268
String	10090.ENSMUSP00000054499
Molecular Weight	Calculated MW: 34.9 kDa Observed MW (SDS-PAGE): 34 kDa
Sequence Fragment	Gly313-Gly607
Sequence	GVVYSVGM QWLKSQGNKQ MLCTCLGNGV SCQETAVTQT YGGNSNGEPC VLPFTYNGRT FYSCTTEGRQ DGHLWCSTTS NYEQDQKYSF CTDHAVLVQT RGGNSNGALC HFPFLYNNRN YTDCTSEGRR DNMKWCGTTQ NYDADQKFGF CPMAAHEEIC TTNEGVMYRI GDQWDKQHDL GHMMRCTCVG NGRGEWACIP YSQLRDQCIV DDITYNVNDT FHKRHEEGHM LNCTCFGQGR GRWKCDPIDQ CQDSETRTFY QIGDSWEKFV HGVRYQCYCY GRGIGEWHCQ PLQTYPG
Tag	N-terminal His tag
Buffer	Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 0.05% Sarcosyl and 5% Trehalose.
Activity	Active
Biological Activity	Fibronectin (FN) is a high-molecular weight (~440 kDa) glycoprotein found in theextracellular matrix that binds to membrane-spanning receptor proteins called integrins. Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Decorin (DCN) has been identified as an interactor of FN, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse FN and recombinant mouse DCN. Briefly, FN was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to DCN-coated microplate wells and incubated for 2 h at 37°C. Wells were washed with PBST and incubated for 1 h with anti-FN polyclonal antibody, then aspirated and washed 3 times. After incubation with HRP-conjugated secondary antibody, wells were aspirated and washed 3 times. TMB substrate solution was added and wells were incubated for 15-25 minutes at 37 °C. Finally, 50 μl stop solution was added to the wells and the absorbance was read at 450 nm immediately. The binding activity of FN and DCN is shown in Figure 3.
Endotoxin Level	< 1.0 EU/µg (LAL method)
Concentration	Prior to lyophilization: 200 µg/ml
Availability	Shipped within 5-7 working days.
Note	THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.

Research Articles on Fibronectin (FN1)

Mouse Fibronectin (FN1) Protein (Active)

Available Options

Documents

Write a review

Research Targets

Service & Support

My Account

Corporate