Mouse Perinuclear Neutrophil Cytoplasmic IgG (MPO-ANCA IgG) ELISA Kit

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Catalogue No: abx054908
Price: US$572.75
(Size: 96 tests)

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Mouse MPO-ANCA IgG ELISA Kit is an ELISA kit against Mouse MPO-ANCA IgG.

Introduction p-ANCA, or MPO-ANCA, or Perinuclear Anti-Neutrophil Cytoplasmic Antibodies, show a perinuclear staining pattern. This pattern occurs because the vast majority of the antigens targeted by ANCAs are highly cationic (Positively charged) at pH 7.00. During ethanol (pH ~7.0 in water) fixation, antigens which are more cationic migrate and localize around the nucleus, attracted by its negatively charged DNA content. Antibody staining therefore results in fluorescence of the region around the nucleus.
Target Perinuclear Neutrophil Cytoplasmic IgG (MPO-ANCA IgG)
Reactivity Mouse
Tested Applications ELISA
Recommended dilutions Optimal dilutions/concentrations should be determined by the end user.
Storage Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual.
Validity The validity for this kit is 6 months.
Stability The stability of the kit is determined by the rate of activity loss. The loss rate is less than 5% within the expiration date under appropriate storage conditions. To minimize performance fluctuations, operation procedures and lab conditions should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same user throughout.
Assay Type Indirect
Assay Data Qualitative
Sample Type Serum and plasma.
Availability Shipped within 5-10 working days.
Note This product is for research use only.

The range and sensitivity is subject to change. Please contact us for the latest product information. For accurate results, sample concentrations must be diluted to mid-range of the kit. If you require a specific range, please contact us in advance or write your request in your order comments.

Please note that our ELISA and CLIA kits are optimised for detection of native samples, rather than recombinant proteins. We are unable to guarantee detection of recombinant proteins, as they may have different sequences or tertiary structures to the native protein.
Plate coated with Antigen
Research Articles on Perinuclear Neutrophil Cytoplasmic IgG (MPO-ANCA IgG)


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