Mouse Perinuclear Neutrophil Cytoplasmic IgG (pANCA IgG) ELISA Kit

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Catalogue No: abx054908
Price: US$572.75
(Size: 96 tests)

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Mouse pANCA IgG ELISA Kit is an ELISA kit against Mouse pANCA IgG.

Introduction p-ANCA, or MPO-ANCA, or Perinuclear Anti-Neutrophil Cytoplasmic Antibodies, show a perinuclear staining pattern. This pattern occurs because the vast majority of the antigens targeted by ANCAs are highly cationic (Positively charged) at pH 7.00. During ethanol (pH ~7.0 in water) fixation, antigens which are more cationic migrate and localize around the nucleus, attracted by its negatively charged DNA content. Antibody staining therefore results in fluorescence of the region around the nucleus.
Target Perinuclear Neutrophil Cytoplasmic IgG (pANCA IgG)
Reactivity Mouse
Tested Applications ELISA
Recommended dilutions Optimal dilutions/concentrations should be determined by the end user.
Storage Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual.
Validity The validity for this kit is 6 months.
Stability The stability of the kit is determined by the rate of activity loss. The loss rate is less than 5% within the expiration date under appropriate storage conditions. To minimize performance fluctuations, operation procedures and lab conditions should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same user throughout.
Assay Type Sandwich
Assay Data Qualitative
Sample Type Serum, plasma and other biological fluids.
Availability Shipped within 5-10 working days.
Note THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES.

Please note that our kits are optimised for detection of native samples, rather than recombinant proteins. We are unable to guarantee detection of recombinant proteins, as they may have different sequences or tertiary structures to the native protein.
Plate coated with Antigen
Research Articles on Perinuclear Neutrophil Cytoplasmic IgG (pANCA IgG)


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