Mitochondrial Ribosomal Protein S30 (MRPS30) Antibody

MRPS30 Antibody is a Rabbit Polyclonal antibody against MRPS30. Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that is similar to the chicken pro-apoptotic protein p52. Transcript variants using alternative promoters or polyA sites have been mentioned in the literature but the complete description of these sequences is not available.

Target	Mitochondrial Ribosomal Protein S30 (MRPS30)
Clonality	Polyclonal
Reactivity	Human, Mouse, Rat
Tested Applications	WB, IF/ICC, IP
Host	Rabbit
Recommended dilutions	WB: 1/500 - 1/2000, IF/ICC: 1/50 - 1/100, IP: 0.5-4 µg/200-400 µg whole cell extracts. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 190-439 of human MRPS30.
Isotype	IgG
Form	Liquid
Purification	Purified by affinity chromatography.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	Q9NP92 (UniProt, ExPASy)
UniProt Secondary AC	Q96I91, Q96Q19, Q9H0P8, Q9NSF9, Q9NZ76
UniProt Entry Name	RT30_HUMAN
Gene Symbol	MRPS30
GeneID	10884
NCBI Accession	NP_057724.2
String	9606.ENSP00000424328
Molecular Weight	Calculated MW: 50 kDa Observed MW: 54 kDa
Buffer	PBS, pH 7.3, containing 0.02% sodium azide, 50% glycerol.
Concentration	> 0.2 mg/ml
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on Mitochondrial Ribosomal Protein S30 (MRPS30)

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