Myc-Tag Antibody

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Catalogue No: abx005583
Price: US$290.00
(Size: 100 µl)

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Western blot analysis of lysates from wild type (WT) and 293F cells transfected with NLK-Myc (C-terminal) or PTEN-Myc (C-terminal) using Mouse anti Myc-Tag antibody at 1/10000 dilution incubated overnight at 4 °C. Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution. Lysates/proteins: 30 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 30s.
Myc-Tag Antibody Myc-Tag Antibody

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Datasheet SDS
Myc-Tag Antibody is a Mouse Monoclonal antibody against Myc-Tag. Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The c-Myc tag corresponds to amino acid residues(EQKLISEEDL) of the human c-Myc protein. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. Myc-Tag mouse mAb detects recombinant proteins containing the Myc tag. The antibody recognizes the Myc-tag EQKLISEEDL fused to either the amino- or carboxy- terminus of targeted proteins.

Target Myc-Tag
Clonality Monoclonal
Reactivity General (All species)
Tested Applications ELISA, WB, IP
Host Mouse
Recommended dilutions ELISA: 1 µg/ml, WB: 1/2000 - 1/6000, IF: 1/1000 - 1/5000, IP: 0.5 µg - 4 µg antibody per 200 µg - 400 µg extracts of whole cells. Optimal dilutions/concentrations should be determined by the end user.
Conjugation Unconjugated
Immunogen A synthetic peptide corresponding to Myc tag.
Isotype IgG1
Form Liquid
Purification Purified by affinity chromatography.
Storage Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
Shelf Life 12 months.
Molecular Weight Observed MW: 50-70 kDa
Buffer PBS, pH 7.3, containing 0.05% Proclin-300, 50% glycerol.
Concentration 2.06 mg/ml
Availability Shipped within 5-10 working days.
Note THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
Research Articles on Myc-Tag


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