Mycoplasma Rapid Detection Kit

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Catalogue No: abx705839
Price: US$203.00
(Size: 20 tests)

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Mycoplasma Rapid Detection Kit

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Abbexa's Mycoplasma Rapid Detection Kit is designed for quick and easy PCR detection of common Mycoplasma species, including Mycoplasma arginini, Mycoplasma hyorhinis, Mycoplasma orale, Mycoplasma pulmonis, Mycoplasma bovis and Mycoplasma yeatsii, in cell culture medium and serum samples.

Kit Components

Component Volume (20 tests) Volume (50 tests)Volume (100 tests)
2X PCR Master Mix (cotaining dye) 0.2 ml 0.5 ml1 ml
10 µm Primer Mix 16 µl40 µl80 µl
Positive Control 16 µl40 µl80 µl
Mycoplasma-Free H2O 1 ml1 ml1 ml


Target Mycoplasma Rapid Detection Kit
Research Area Cell Culture
Form Liquid
Storage Store between -5°C and -20°C. Avoid exposure to light.
Shelf Life Up to 24 months.
Availability Shipped within 5-7 working days.
Note THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
Directions for use Recommended PCR Procedure:
  1. Culture cells for 48-72 hours or until 80% confluent. It is recommended to take 10 µl culture medium as a backup for when the cell confluence exceeds 80%.
  2. Suspend the cells,and centrifuge for 3 minutes.
  3. Create a reaction mixture using the following reagents. It is recommended to multiply each of these volumes by the number of reactions being carried out. Ensure the reagents are at room temperature before use and mix well.

    Component Test Sample Positive ControlNegative Control
    2X PCR Master Mix (cotaining dye) 10 µl 10 µl10 µl
    Sample 2 µl test sample2 µl positive control-
    10 µM Primer Mix 0.8 µl0.8 µl0.8 µl
    Mycoplasma-Free H2O 7.2 µl7.2 µl9.2 µl

  4. Set up the PCR machine and allow it to warm up. Ensure all PCR tubes are covered tightly before placing them in the machine.
  5. Program the appropriate PCR cycling protocol on your RT-PCR instrument. A suggested thermal cycle is below:

    6. StepTemperatureTimeCycle
    194°C3 minutes1
    294°C15 seconds35-40
    360°C15 seconds
    472°C15 seconds
    572°C1 minute1
    64°C--

  6. After PCR, detection should be carried out as soon as possible. The product can be stored at 4°C for up to 1 week.

Gel Electrophoresis
  1. Use 10 µl PCR product and 1-1.5% agarose gel (at 160 V for 15 minutes).
  2. Compare the results with the positive and negative control samples. The positive band should be approximately 500 bp with slight fluctiation depending on the species.

Notes:
  • It is recommended to centrifuge all components before use at 1000 rpm for 30 seconds to mix and ensure the components are at the bottom of the tube
  • After creating the reaction mixture it should be used as soon as possible. Store at 4°C and complete the reaction within 6 hours.
  • Test the sample as soon as possible, and within 48 hours. or store at -5°C to -20°C for up to 1 month.
  • Ensure all liquid handling is done under sterile conditions, it is recommended to use a tissue culture hood. The use of PPE such as gloves, a lab coat and a mask is recommended to avoid contamination.
  • For samples with weak band, centrifuge at 1200 rpm for 3 minutes to remove the supernatant, dilute the sample 1/10 with Mycoplasma-Free water and re-test.
  • Discard all samples/reagents if they are not used within the recommended shelf life.
Research Articles on Mycoplasma Rapid Detection Kit


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