Myosin-9 (MYH9) Antibody

MYH9 Antibody is a Rabbit Polyclonal antibody against MYH9. Nonmuscle myosin is an actin-based motor protein essential to cell motility, cell division, migration, adhesion, and polarity. The holoenzyme consists of two identical heavy chains and two sets of light chains. The light chains (MLCs) regulate myosin II activity and stability. The heavy chains (NMHCs) are encoded by three genes, MYH9, MYH10, and MYH14, which generate three different nonmuscle myosin II isoforms, IIa, IIb, and IIc, respectively (reviewed in 1). While all three isoforms perform the same enzymatic tasks, binding to and contracting actin filaments coupled to ATP hydrolysis, their cellular functions do not appear to be redundant and they have different subcellular distributions (2-5). The carboxy-terminal tail domain of myosin II is important in isoform-specific subcellular localization (6). Phosphorylation of myosin IIa at Ser1943 contributes to the regulation of breast cancer cell migration (7).

Target	Myosin-9 (MYH9)
Clonality	Polyclonal
Reactivity	Human, Mouse
Tested Applications	WB, IHC
Host	Rabbit
Recommended dilutions	WB: 1/1000 - 1/2000, IHC-P: 1/50 - 1/100. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1711-1960 of human MYH9.
Isotype	IgG
Form	Liquid
Purification	Purified by affinity chromatography.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	P35579 (UniProt, ExPASy)
UniProt Secondary AC	A8K6E4, O60805, Q60FE2, Q86T83
UniProt Entry Name	MYH9_HUMAN
Gene Symbol	MYH9
GeneID	4627
OMIM	155100
NCBI Accession	NP_002464.1 NM_002473.5
HGNC	7579
KEGG	hsa:4627
Ensembl	ENSG00000100345
String	9606.ENSP00000216181
Molecular Weight	Calculated MW: 227 kDa Observed MW: 250 kDa
Buffer	PBS, pH 7.3, containing 0.02% sodium azide, 50% glycerol.
Concentration	> 0.2 mg/ml
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on Myosin-9 (MYH9)

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