Nestin (NES) Antibody

Nestin (NES) Antibody is a Rabbit Polyclonal antibody for the detection of Nestin.

Nestin, also named as NES and Nbla00170, is a class VI intermediate filament protein expressed in stem cells of the central nervous system (CNS) but not in mature CNS cells. Its expression is used extensively as a marker for neural lineage cells derived from human embryonic stem (hES) and induced pluripotent stem (iPS) cells. Nestin is also expressed in non-neural stem cell populations such as pancreatic islet progenitors and hematopoietic progenitors.

Target	Nestin (NES)
Clonality	Polyclonal
Reactivity	Human, Mouse, Rat
Tested Applications	ELISA, WB, IHC, IF/ICC
Host	Rabbit
Recommended dilutions	WB: 1/500 - 1/2000, IHC: 1/500 - 1/200, IF/ICC: 1/20 - 1/200. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	nestin
Isotype	IgG
Form	Liquid
Purity	≥ 95% (SDS-PAGE)
Purification	Purified by immunogen affinity chromatography.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
Validity	12 months.
UniProt Primary AC	P48681 (UniProt, ExPASy)
UniProt Secondary AC	O00552, Q3LIF5, Q5SYZ6
UniProt Entry Name	NEST_HUMAN
Gene Symbol	NES
GeneID	10763
OMIM	600915
HGNC	7756
KEGG	hsa:10763
Ensembl	ENSG00000132688
String	9606.ENSP00000357206
Molecular Weight	Observed MW: 170 kDa
Buffer	PBS, pH 7.3, with 0.02% sodium azide and 50% glycerol.
Concentration	2 mg/ml
Availability	Shipped within 5-12 working days.
Note	This product is for research use only.

Research Articles on Nestin (NES)

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