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Human Sialidase-1 (NEU1) Protein
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Catalogue No: abx651422
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US$797.50
(Size: 50 µg)
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Datasheet SDS
Human Sialidase-1 (NEU1) Protein is an active Human protein expressed in E coli. This protein exhibits binding activity as detailed in the Biological Activity section below. Other types of biological activity, such as enzymatic activity, have not been tested.
Research Articles on Sialidase-1 (NEU1)
| Target | Sialidase-1 (NEU1) |
| Research Area | Enzymes and Kinases, Tumor Immunity, Infection Immunity, Neuroscience |
| Origin | Human |
| Expression | Recombinant |
| Tested Applications | WB, SDS-PAGE |
| Host | E. coli |
| Conjugation | Unconjugated |
| Form | Lyophilized |
| Activity | Exhibits binding activity. Enzymatic activity has not been tested. |
| Biological Activity | A binding ELISA assay was conducted to detect the interaction of recombinant Human NEU and recombinant human CTSA. Briefly, NEU was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to CTSA-coated microplate wells and incubated for 2 h at 37 °C. Wells were washed with PBST and incubated for 1 h with anti-NEU polyclonal antibody, then aspirated and washed 3 times. After incubation with HRP-conjugated secondary antibody, wells were aspirated and washed 3 times. TMB substrate solution was added and wells were incubated for 15-25 minutes at 37 °C. Finally, 50 μl stop solution was added to the wells and the absorbance was read at 450 nm immediately. The binding activity of NEU and CTSA is shown in the figures. A dose-dependent effect was observed. |
| Purity | > 80% |
| Reconstitution | Reconstitute in ddH2O to a concentration ≤ 0.1 mg/ml. Do not vortex. |
| Storage | Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze–thaw cycles. |
| Shelf Life | 12 months. |
| UniProt Primary AC | Q99519 (UniProt, ExPASy) |
| UniProt Entry Name | NEUR1_HUMAN |
| Gene Symbol | NEU1 |
| GeneID | 4758 |
| KEGG | hsa:4758 |
| String | 9606.ENSP00000364782 |
| Molecular Weight | Calculated MW: 45.0 kDa Observed MW (SDS-PAGE): 46 kDa |
| Sequence Fragment | Ala47-Leu415 |
| Sequence | AEND FGLVQPLVTM EQLLWVSGRQ IGSVDTFRIP LITATPRATL LAFAEARKMS SSDEGAKFIA LRRSMDQGST WSPTAFIVND GDVPDGLNLG AVVSDVETGV VFLFYSLCAH KAGCQVASTM LVWSKDDGVS WSTPRNLSLD IGTEVFAPGP GSGIQKQREP RKGRLIVCGH GTLERDGVFC LLSDDHGASW RYGSGVSGIP YGQPKQENDF NPDECQPYEL PDGSVVINAR NQNNYHCHCR IVLRSYDACD TLRPRDVTFD PELVDPVVAA GAVVTSSGIV FFSNPAHPEF RVNLTLRWSF SNGTSWRKET VQLWPGPSGY SSLATLEGSM DGEEQAPQLY VLYEKGRNHY TESISVAKIS VYGTL |
| Tag | N-terminal His tag |
| Buffer | Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 5% Trehalose. |
| Endotoxin Level | < 1.0 EU/µg protein (LAL method) |
| Concentration | Prior to lyophilization: 80 µg/ml |
| Availability | Shipped within 5-7 working days. |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. |