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| Target | Neurocan (NCAN) |
| Origin | Mouse |
| Expression | Recombinant |
| Tested Applications | WB, SDS-PAGE |
| Host | E. coli |
| Conjugation | Unconjugated |
| Form | Lyophilized |
| Activity | Not tested |
| Purity | > 97% |
| Reconstitution | Reconstitute in ddH2O to a concentration between 0.1-1.0 mg/ml. Do not vortex. |
| Storage | Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze–thaw cycles. |
| Shelf Life | 12 months. |
| UniProt Primary AC | P55066 (UniProt, ExPASy) |
| UniProt Secondary AC | Q6P1E3, Q8C4F8 |
| UniProt Entry Name | NCAN_MOUSE |
| Gene Symbol | NCAN |
| GeneID | 13004 |
| KEGG | mmu:13004 |
| String | 10090.ENSMUSP00000002412 |
| Molecular Weight | Calculated MW: 33.8 kDa Observed MW (SDS-PAGE): 38 kDa Possible reasons why the actual band size differs from the predicted band size: 1. Splice variants. Alternative splicing may create different sized proteins from the same gene. 2. Relative charge. The composition of amino acids may affect the charge of the protein. 3. Post-translational modification. Phosphorylation, glycoslyation, methylation etc. may affect the band size. 4. Post-translational cleavage. Many proteins are synthesised as pro-proteins, and then cleaved to give the active form. 5. Polymerisation of the target protein. Dimerisation, multimerisation etc. will increase the band size observed. |
| Sequence Fragment | Pro960-Lys1225 |
| Sequence | P TDPCENNPCL HGGTCHTNGT VYGCSCDQGY AGENCEIDID DCLCSPCENG GTCIDEVNGF ICLCLPSYGG SLCEKDTEGC DRGWHKFQGH CYRYFAHRRA WEDAERDCRR RAGHLTSVHS PEEHKFINSF GHENSWIGLN DRTVERDFQW TDNTGLQYEN WREKQPDNFF AGGEDCVVMV AHESGRWNDV PCNYNLPYVC KKGTVLCGPP PAVENASLVG VRKIKYNVHA TVRYQCDEGF SQHRVATIRC RNNGKWDRPQ IMCIK |
| Tag | N-terminal His tag |
| Buffer | Prior to lyophilization: 100 mM NaHCO3, 500 mM NaCl, pH 8.3, containing 0.01% Sarcosyl, 5% Trehalose. |
| Concentration | Prior to lyophilization: 150 µg/ml |
| Availability | Shipped within 5-7 working days. |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. |