NPHS1, Nephrin (NPHS1) Antibody

NPHS1 Antibody is a Rabbit Polyclonal antibody against NPHS1. This gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. The gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine. Mutations in this gene result in Finnish-type congenital nephrosis 1, characterized by severe proteinuria and loss of the slit diaphragm and foot processes. In Western blots, nephrin antibodies generated against the two terminal extracellular Ig domains of recombinant human nephrin recognized a 180-kDa protein in lysates of human glomeruli and a 150-kDa protein in transfected COS-7 cell lysates.

Target	NPHS1, Nephrin (NPHS1)
Clonality	Polyclonal
Reactivity	Mouse, Rat
Tested Applications	ELISA, WB
Host	Rabbit
Recommended dilutions	ELISA: 1 µg/ml, WB: 1/500 - 1/1000. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1019-1241 of human NPHS1.
Isotype	IgG
Form	Liquid
Purification	Purified by affinity chromatography.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	O60500 (UniProt, ExPASy)
UniProt Secondary AC	A6NDH2, C3RX61
UniProt Entry Name	NPHN_HUMAN
Gene Symbol	NPHS1
GeneID	4868
NCBI Accession	NP_004637.1
KEGG	hsa:4868
String	9606.ENSP00000368190
Molecular Weight	Calculated MW: 135 kDa Observed MW: 130 kDa
Buffer	PBS, pH 7.3, containing 0.05% Proclin-300, 50% glycerol.
Concentration	> 0.2 mg/ml
Availability	Shipped within 5-10 working days.
Note	THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.

Research Articles on NPHS1, Nephrin (NPHS1)

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