5'-Nucleotidase / CD73 (NT5E) Antibody

5'-nucleotidase, ecto (NT5E), also known as CD73 (Cluster of Differentiation 73).Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase; EC 3.1.3.5) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP.The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane.The enzyme is used as a marker of lymphocyte differentiation.Consequently, a deficiency of NT5 occurs in a variety of immunodeficiency diseases (e.g., see MIM 102700, MIM 300300).Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate.

Target	5'-Nucleotidase / CD73 (NT5E)
Clonality	Monoclonal
Clone	W565
Reactivity	Human
Tested Applications	ELISA, IHC
Host	Mouse
Recommended dilutions	ELISA: 1/10000, IHC: 1/200 - 1/1000. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	Purified recombinant fragment of NT5E expressed in E. coli.
Isotype	IgG1
Form	Liquid
Purification	Unpurified ascites.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	P21589 (UniProt, ExPASy)
UniProt Secondary AC	B3KQI8, O75520, Q5W116
UniProt Entry Name	5NTD_HUMAN
Gene Symbol	NT5E
GeneID	4907
OMIM	129190
HGNC	8021
KEGG	hsa:4907
Ensembl	ENSG00000135318
String	9606.ENSP00000257770
Molecular Weight	70 kDa
Buffer	Ascitic fluid containing 0.03% sodium azide.
Concentration	Not determined.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on 5'-Nucleotidase / CD73 (NT5E)

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