P16 (Mouse and Human) Antibody

The progression of cells through the cell cycle is regulated by a family of protein kinases known as cyclin-dependent kinases (Cdks).The sequential activation of individual members of this family and their consequent phosphorylation of critical substrates promotes orderly progression through the cell cycle.The cyclins function as differentially expressed positive regulators of Cdks.Negative regulators of the cycle include the p53-inducible 21 kDa WAF1/Cip1 protein designated p21, Kip1 p27 and p16.The complexes formed by Cdk4 and the D-type cyclins have been strongly implicated in the control of cell proliferation during the G1 phase.It has recently been shown that p16 binds to Cdk4 and inhibits the catalytic activity of the Cdk4/cyclin D complex.Moreover, the gene encoding p16 exhibits a high frequency of homozygous deletions and point mutations in established human tumor cell lines.

Target	P16 (Mouse and Human)
Clonality	Monoclonal
Reactivity	Human, Rat
Tested Applications	ELISA, IHC
Host	Mouse
Recommended dilutions	ELISA: 1/10000, IHC: 1/200 - 1/1000. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	Purified recombinant fragment of P16 expressed in E. coli.
Isotype	IgG2b
Form	Liquid
Purification	Unpurified ascites.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
GeneID	1029
Buffer	Ascitic fluid containing 0.03% sodium azide.
Concentration	Not determined.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on P16 (Mouse and Human)

Write a review

Your Name

Your Email Address

PMID

DOI

Your Review

Rating

Continue