p53 (pS315) Antibody

Tumor protein p53, a nuclear protein, plays an essential role in the regulation of cell cycle, specifically in the transition from G0 to G1. It is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing DNA-binding, oligomerization and transcription activation domains. It is postulated to bind as a tetramer to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Alterations of the TP53 gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome.

Target	p53 (pS315)
Clonality	Polyclonal
Target Modification	Ser315
Modification	Phosphorylation
Reactivity	Human
Tested Applications	ELISA, IHC, DB
Host	Rabbit
Recommended dilutions	IHC-P: 1/50 - 1/100, DB: 1/500. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	KLH-conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S315 of human p53.
Isotype	IgG
Form	Liquid
Purification	Purified by protein G affinity chromatography. Then, the antibody fraction was peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody was eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	P04637 (UniProt, ExPASy)
UniProt Entry Name	P53_HUMAN
KEGG	hsa:7157
String	9606.ENSP00000269305
Molecular Weight	Calculated MW: 43.7 kDa
Buffer	PBS containing 0.09% sodium azide.
Specificity	Predicted to react with Monkey TP53.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on p53 (pS315)

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