Rat Parathyroid Hormone (PTH) Protein

Parathyroid Hormone (PTH) Protein (Active) is an active protein from Rat.

Target	Parathyroid Hormone (PTH)
Origin	Rat
Tested Applications	WB, SDS-PAGE
Conjugation	Unconjugated
Form	Lyophilized
Purity	> 92%
Reconstitution	To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH₂O. If a lower concentration is required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0. If a higher concentration is required, the product can be reconstituted directly in 20 mM Tris, 150 mM NaCl, pH 8.0, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
Storage	Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	P04089 (UniProt, ExPASy)
UniProt Secondary AC	Q63473
UniProt Entry Name	PTHY_RAT
KEGG	rno:24694
String	10116.ENSRNOP00000019199
Molecular Weight	Calculated MW: 59.4 kDa Observed MW (SDS-PAGE): 59 kDa
Sequence Fragment	Ala32-Gln115
Sequence	AVSEIQLMH NLGKHLASVE RMQWLRKKLQ DVHNFVSLGV QMAAREGSYQ RPTKKEENVL VDGNSKSLGE GDKADVDVLV KAKSQ
Tag	N-terminal His tag and MBP tag
Buffer	Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01% Sarcosyl, 5% Trehalose and Proclin-300.
Activity	Active
Biological Activity	Parathyroid Hormone (PTH) is a hormone secreted by the parathyroid glands, and is important in bone remodeling. Osteoblast-like cell lines that express Parathyroid Hormone 1 Receptor (PTHR1), such as ROS 17/2.8, UMR106, SaOS, U2OS, and MG63, show increased proliferation when exposed to PTH. Rat PTH shares similarities with human PTH; the homology based on amino acid sequence is 71.3%. Therefore, a proliferation assay of recombinant Rat PTH was conducted using U2OS cells. Briefly, U2OS cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of PTH. After incubation for 48 h, cells were observed using an inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 µl of CCK-8 solution was added to each well of the plate, which was incubated for 1-4 hours at 37°C. The absorbance was then measured at 450 nm using a microplate reader. Cell proliferation of U2OS cells after incubation with PTH for 48 h observed by inverted microscope is shown in Figure 3 and Figure 4. Cell viability assessed by CCK-8 is shown in Figure 5, where it can be seen that Rat PTH increased cell viability of U2OS cells.
Concentration	Prior to lyophilization: 200 µg/ml
Availability	Shipped within 5-7 working days.
Note	This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use.

Research Articles on Parathyroid Hormone (PTH)

Rat Parathyroid Hormone (PTH) Protein

Available Options

Documents

Write a review

Research Targets

Service & Support

My Account

Corporate