PCR SuperMix (-dye)

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Catalogue No: abx098002
Price: US$275.50
(Size: 1 ml)
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PCR SuperMix (-dye)

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Datasheet
PCR SuperMix is a ready-to-use mixture of DNA Polymerase, dNTPs and optimized buffer. The supermix is provided at 2x concentration and used at 1x concentration by adding the template, primers and nuclease-free water. PCR products are unsuitable for PAGE. The Extension rate is about 1-2 kb/min. Amplification of genomic DNA fragment up to 4 kb. Contents:

Component 1 ml size 5 ml size 15 ml size
2X PCR SuperMix (-dye) 1 ml 5 × 1 ml 15 × 1 ml
ddH2O 1 ml 5 ml 3 × 5 ml


Target PCR SuperMix (-dye)
Storage Store at -20°C. Stable for 12 months from date of receipt.
Availability Shipped within 10-20 working days.
Note This product is for research use only.
Directions for use Reaction Components:

Component Volume Final Concentration
Template Variable as required
Forward Primer (10 µM) 1 µl 0.2 µM
Reverse Primer (10 µM) 1 µl 0.2 µM
2X PCR SuperMix 25 µl 1X
ddH2O Variable N/A
Total Volume 50 µl N/A

Thermal Cycling Conditions:
  1. 94 °C, 2-5 min
  2. 94 °C, 20 s
  3. 50-60 °C, 20 s
  4. 72 °C, 2-6 kb/min
  5. Repeat 2-4 for 30-35 cycles.
  6. 72 °C, 5-10 min
Notes:

Before use, completely thaw all components and mix well.
Research Articles on PCR SuperMix (-dye)


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