During tissue and cells lysis, a large number of endogenous proteins are released, including phosphatases. Phosphatases catalyse dephosphorylation of phosphorylated proteins. This product contains 3 independent protein phosphatase inhibitors, each of which can specifically inhibit the activity of one or several protein phosphatases. This product inhibits the activity of almost all main protein phosphatases, including protein serine / threonine phosphatases (PP1, PP2A, PP2B, PP2C), protein tyrosine phosphatases (PTPs), alkaline phosphatase, acid phosphatase etc. This product can be added to tissues and cell lysis products to maintain the phosphorylated state of any phosphorylated proteins present. It can be used to detect phosphorylated proteins, protein kinase activity, etc in Western Blot and Co-Immunoprecipitation. This product is provided as a 100X concentrated solution.
Tested Applications
WB
Storage
Store at -20°C.
Shelf Life
12 months.
Availability
Shipped within 5-10 working days.
Note
THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
Directions for use
Dilute 1:100 in an appropriate lysis buffer and mix thoroughly.
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