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Protein Kinase, cAMP Dependent Catalytic Alpha/Beta Phospho-Thr197 (PRKACA/PRKACB pT197) Antibody
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Catalogue No: abx012448
Price:
US$50.75
(Size: 10 µg)
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Rabbit polyclonal antibody against PKA alpha/beta CAT protein when phosphorylated at Thr197. Immunogen region is Internal. Specificity is as follows for the reactive species: H:T198, M:T198, R:T198.
Research Articles on Protein Kinase, cAMP Dependent Catalytic Alpha/Beta Phospho-Thr197 (PRKACA/PRKACB pT197)
| Target | Protein Kinase, cAMP Dependent Catalytic Alpha/Beta Phospho-Thr197 (PRKACA/PRKACB pT197) |
| Clonality | Polyclonal |
| Target Modification | Thr197 |
| Modification | Phosphorylation |
| Reactivity | Human, Mouse, Rat |
| Tested Applications | ELISA, WB, IHC, IF/ICC |
| Host | Rabbit |
| Recommended dilutions | WB: 1/500 - 1/3000, IHC: 1/50 - 1/100, IF/ICC: 1/100 - 1/500, ELISA: 1/10000. Optimal dilutions/concentrations should be determined by the end user. |
| Conjugation | Unconjugated |
| Immunogen | The antiserum was produced against synthesized phosphopeptide derived from human PKA alpha/beta CAT around the phosphorylation site of threonine 197 (T-W-TP-L-C). |
| Isotype | IgG |
| Form | Liquid |
| Purification | Purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against the non-phosphopeptide was removed by chromatography using the non-phosphopeptide corresponding to the phosphorylation site. |
| Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| UniProt Primary AC | P17612 (UniProt, ExPASy) P22694 (UniProt, ExPASy) |
| UniProt Entry Name | KAPCA_HUMAN, KAPCB_HUMAN |
| Sequence | VKGRTWTLCGTPEY |
| Buffer | PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% sodium azide, 50% glycerol. |
| Specificity | Detects endogenous levels of PKA alpha/beta CAT only when phosphorylated at threonine 197. |
| Concentration | 1 mg/ml |
| Availability | Shipped within 5-10 working days. |
| Note | This product is for research use only. |
