RNA Polymerase I Subunit A (POLR1A) Antibody

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol I. A bridging helix emanates from RPA1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol I by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition (By similarity).

Target	RNA Polymerase I Subunit A (POLR1A)
Clonality	Polyclonal
Reactivity	Human
Tested Applications	ELISA, WB
Host	Rabbit
Recommended dilutions	WB: 1/1000. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	KLH-conjugated synthetic peptide between 1437-1465 amino acids from the C-terminal region of human POLR1A.
Isotype	IgG
Form	Liquid
Purification	Purified through a protein A column, followed by peptide affinity purification.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	O95602 (UniProt, ExPASy)
UniProt Secondary AC	B7Z7T0, D6W5M0, Q0VG05, Q9UEH0, Q9UFT9
UniProt Entry Name	RPA1_HUMAN
Gene Symbol	POLR1A
KEGG	hsa:25885
String	9606.ENSP00000263857
Molecular Weight	Calculated MW: 195 kDa
Buffer	PBS containing 0.09% sodium azide.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on RNA Polymerase I Subunit A (POLR1A)

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