DNA-Directed RNA Polymerase II Subunit RPB1 (POLR2A) Antibody

POLR2A Antibody is a Rabbit Polyclonal antibody against POLR2A. This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.

Target	DNA-Directed RNA Polymerase II Subunit RPB1 (POLR2A)
Clonality	Polyclonal
Reactivity	Human, Mouse, Rat
Tested Applications	WB, IF/ICC, IP
Host	Rabbit
Recommended dilutions	WB: 1/500 - 1/2000, IF/ICC: 1/50 - 1/200, IP: 0.5-4 µg/200-400 µg whole cell extracts. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human POLR2A.
Isotype	IgG
Form	Liquid
Purification	Purified by affinity chromatography.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	P24928 (UniProt, ExPASy)
UniProt Secondary AC	A6NN93, B9EH88, Q6NX41
UniProt Entry Name	RPB1_HUMAN
Gene Symbol	POLR2A
GeneID	5430
OMIM	180660
NCBI Accession	NP_000928.1
HGNC	9187
KEGG	hsa:5430
Ensembl	ENSG00000181222
String	9606.ENSP00000480158
Molecular Weight	Calculated MW: 217 kDa Observed MW: 210-250 kDa
Buffer	PBS, pH 7.3, containing 0.01% thimerosal, 50% glycerol.
Concentration	> 0.2 mg/ml
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on DNA-Directed RNA Polymerase II Subunit RPB1 (POLR2A)

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