Human Poly ADP Ribose Polymerase 1 (PARP1) Protein

Human Poly ADP Ribose Polymerase 1 Protein is a recombinant Human protein expressed in E. coli.

This protein is the immunogen for the following antibodies: abx128898, abx174131

Target	Poly ADP Ribose Polymerase 1 (PARP1)
Origin	Human
Expression	Recombinant
Tested Applications	WB, SDS-PAGE
Host	E. coli
Conjugation	Unconjugated
Form	Lyophilized
Purity	> 95%
Reconstitution	To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH₂O. If a lower concentration is required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0. If a higher concentration is required, the product can be reconstituted directly in 20 mM Tris, 150 mM NaCl, pH 8.0, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
Storage	Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	P09874 (UniProt, ExPASy)
UniProt Secondary AC	B1ANJ4, Q8IUZ9
UniProt Entry Name	PARP1_HUMAN
Gene Symbol	PARP1
GeneID	142
KEGG	hsa:142
String	9606.ENSP00000355759
Molecular Weight	Calculated MW: 29.3 kDa Observed MW (SDS-PAGE): 32 kDa
Sequence Fragment	Asp788-Trp1014
Sequence	DPI DVNYEKLKTD IKVVDRDSEE AEIIRKYVKN THATTHNAYD LEVIDIFKIE REGECQRYKP FKQLHNRRLL WHGSRTTNFA GILSQGLRIA PPEAPVTGYM FGKGIYFADM VSKSANYCHT SQGDPIGLIL LGEVALGNMY ELKHASHISK LPKGKHSVKG LGKTTPDPSA NISLDGVDVP LGTGISSGVN DTSLLYNEYI VYDIAQVNLK YLLKLKFNFK TSLW
Tag	N-terminal His tag
Buffer	Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01% Sarcosyl, 5% Trehalose and Proclin-300.
Activity	Not tested
Concentration	Prior to lyophilization: 200 µg/ml
Availability	Shipped within 5-7 working days.
Note	This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use.

Research Articles on Poly ADP Ribose Polymerase 1 (PARP1)

Human Poly ADP Ribose Polymerase 1 (PARP1) Protein

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