(Size: 50 µl)
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Prestained protein ladders / marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verifying Western transfer efficiency on PVDF, nylon and nitrocellulose membranes. It is also used to approximate sizing of proteins on SDS-PAGE and Western blots. It is composed of 6 prestained proteins of 3 different colors, with molecular weights from 1.7 kDa to 40 kDa.
Research Articles on 1.7-40 kDa Protein Marker (Stained)
||1.7-40 kDa Protein Marker (Stained)
||Store at 4°C for up to 3 months. For long-term storage, store at -20°C.
||62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3 and 33% glycerol.
|Directions for use
- Thaw the ladder either at room temperature or at 37 °C for a few minutes to dissolve precipitated solids thoroughly. Do not boil.
- Mix gently but thoroughly, to ensure that the solution is homogeneous.
- Load the following volumes of the ladder:
Small/Mini SDS-polyacrylamide gel: 5 µl per well (0.75-1.0 mm thickness)
Large SDS-polyacrylamide gel: 10 µl per well (0.75-1.0 mm thickness)
||Shipped within 5-10 working days.
||This product is for research use only.