Protein Kinase cAMP-Dependent Type II Regulatory Subunit Alpha (PRKAR2A) Antibody
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Catalogue No: abx001289
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US$232.00
(Size: 20 µl)
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(![Western blot analysis of lysates from wild type (WT) and PKA RIIα (PRKAR2A)/PKR2 knockout (KO) HeLa cells, using [KO Validated] PKA RIIα (PRKAR2A) Antibody at 1/1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1/10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 5s.](https://www.abbexa.com/image/cache/data/products/18B/abx001289_1-500x500.jpg "Protein Kinase cAMP-Dependent Type II Regulatory Subunit Alpha (PRKAR2A) Antibody")
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PRKAR2A Antibody is a Rabbit Polyclonal antibody against PRKAR2A. The effects of cAMP in most tissues and cell types are mainly modulated via protein kinase A, a heterotetrameric protein complex consisting of two regulatory ® and two catalytic © subunits. The regulatory subunit of cAMP-dependent protein kinase (PRKAR2A) is one of the regulatory subunits and the gene is located on chromosome region 3p21.3-p21.2. The expression of PRKAR2A is tightly regulated during spermatogenesis, a significant increase in expression of this gene was also found in the human myometrium during pregnancy. This antibody got two bands about 45 kDa and 50-55 kDa in western blotting, and the 50-55kDa may be caused by phosphorylation.
Research Articles on Protein Kinase cAMP-Dependent Type II Regulatory Subunit Alpha (PRKAR2A)
| Target | Protein Kinase cAMP-Dependent Type II Regulatory Subunit Alpha (PRKAR2A) |
| Clonality | Polyclonal |
| Reactivity | Human, Mouse, Rat |
| Tested Applications | ELISA, WB, IF/ICC |
| Host | Rabbit |
| Recommended dilutions | ELISA: 1 µg/ml, WB: 1/1000 - 1/2000, IF/ICC: 1/50 - 1/200. Optimal dilutions/concentrations should be determined by the end user. |
| Conjugation | Unconjugated |
| Immunogen | Recombinant protein corresponding to PRKAR2A. The exact sequence is proprietary. |
| Isotype | IgG |
| Form | Liquid |
| Purification | Purified by affinity chromatography. |
| Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| Shelf Life | 12 months. |
| UniProt Primary AC | P13861 (UniProt, ExPASy) |
| UniProt Secondary AC | Q16823, Q9BUB1 |
| UniProt Entry Name | KAP2_HUMAN |
| Gene Symbol | PRKAR2A |
| GeneID | 5576 |
| NCBI Accession | NP_004148.1 |
| KEGG | hsa:5576 |
| String | 9606.ENSP00000265563 |
| Molecular Weight | Calculated MW: 46 kDa Observed MW: 46 kDa |
| Buffer | PBS, pH 7.3, containing 0.09% sodium azide, 50% glycerol. |
| Concentration | > 0.2 mg/ml |
| Availability | Shipped within 5-10 working days. |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. |