Prosaposin (PSAP) Antibody Pair

REQUEST MORE INFO
Catalogue No: abx370510
Price: US$1,551.50
(Size: 5 × 96 tests)

Orders to United States ship from United States

Reviews: There are no reviews for this product.<br>Leave a review for us and help us better the community and our services.
(There are no reviews for this product.
Leave a review for us and help us better the community and our services.)
Prosaposin (PSAP) Antibody Pair

Click on the image to see the image legend

Available Options

* Size:


Add to Cart Buy It Now GET A QUOTE

Documents

Datasheet SDS
Prosaposin (PSAP) Antibody Pair for use in Sandwich ELISA assay development. This antibody pair contains:

Component 5 × 96 tests 10 × 96 tests
Capture Antibody200 µg 400 µg
Biotin-Conjugated Detection Antibody 50 µg 100 µg
Standard 2 µg 10 µg

Please note that quantities and concentrations may change between different batches.

It is recommended to use this antibody pair with abx098958 Antibody Pair Support Kit (Sandwich Method).

Target Prosaposin (PSAP)
Research Area Metabolism
Reactivity Human
Tested Applications ELISA
Recommended dilutions Dilute the Capture Antibody 125-fold with Coating Buffer.
Dilute the Biotin-Conjugated Detection Antibody 200-fold with Detection Antibody Diluent.

Optimal dilutions/concentrations should be determined by the end user.
Form Liquid (Capture Antibody and Detection Antibody)
Reconstitution Reconstitute the standard with Standard Diluent. The volume, and therefore standard concentration, should be determined by the end user. For a detection range of 0.156 ng/ml - 10 ng/ml, a reconstitution volume of 1.0 ml is recommended.
Storage Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
Shelf Life 12 months.
UniProt Primary AC P07602 (UniProt, ExPASy)
UniProt Secondary AC P07292, P15793, P78538, P78541, P78546, P78547, P78558, Q53Y86, Q6IBQ6, Q92739, Q92740, Q92741, Q92742
UniProt Entry Name SAP_HUMAN
Gene Symbol PSAP
GeneID 5660
OMIM 176801
HGNC 9498
KEGG hsa:5660
Ensembl ENSG00000197746
String 9606.ENSP00000378394
Buffer The Capture and Detection Antibody both contain 0.1% sodium azide.
Test Range 0.156 ng/ml - 10 ng/ml
Standard Form Lyophilized
Assay Type Sandwich
Capture Antibody Host Rabbit
Detection Antibody Host Rabbit
Capture Antibody Clonality Polyclonal
Detection Antibody Clonality Polyclonal
Capture Antibody Conjugation Unconjugated
Detection Antibody Conjugation Biotin
Concentration Capture Antibody: 0.5 mg/ml
Biotin-Conjugated Detection Antibody: 0.2 mg/ml
Availability Shipped within 5-20 working days.
Note THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
Directions for use Bring all components to room temperature (18-25°C) and briefly spin or centrifuge the vials before use. Working solutions should be prepared and used immediately.

Recommended Procedure:

  1. Dilute the Capture Antibody to working concentration using Coating Buffer. Immediately coat the 96-well plate with diluted Capture Antibody (100 µl per well). Seal the plate and incubate at 4 °C overnight or at 37 °C for 2 hours
  2. Aspirate the wells and wash with Wash Buffer (350 µl per well) and allow to soak for 1-2 min. Remove the liquid by inverting and tapping the plate on to absorbent paper.
  3. Block the plate with Blocking Buffer (200 µl per well) at 37 °C for 1.5 hours.
  4. Repeat the aspiration/wash process in Step 2.
  5. Add 100 µl of standards or sample into the appropriate wells. Cover with a plate sealer and incubate at 37 °C for 1 hour.
  6. Repeat the aspiration/wash process in Step 2.
  7. Add appropriately diluted Biotin-Conjugated Detection Antibody (100 µl per well). Cover the plate with a new plate sealer and incubate at 37 °C for 1 hour.
  8. Repeat the aspiration/wash process in Step 2.
  9. Add appropriately diluted Streptavidin HRP (100 µl per well). Cover the plate with a new plate sealer and incubate at 37 °C for 30 min.
  10. Repeat the aspiration/wash process in Step 2.
  11. Add Substrate Solution (90 µl per well). Cover the plate with a new plate sealer and incubate at 37 °C for 10-20 min. Keep the plate in the dark and avoid exposure to light.
  12. Add Stop Solution (50 µl per well). Tap the side of the plate to ensure thorough mixing.
  13. Measure the absorbance immediately using a microplate reader set at 450 nm.
Research Articles on Prosaposin (PSAP)


Write a review

Continue
Tags:
(Click to show)