Protein G Resin

REQUEST MORE INFO
Catalogue No: abx098112
Price: US$638.00
(Size: 5 ml)

Click on the image to see the image legend

Available Options

* Size:

Add to Shopping Cart Buy It Now GET A QUOTE

Documents

Datasheet
Protein G Resin is an affinity chromatography resin with high binding capacity for IgG. Protein G Resin is suitable for purification of monoclonal antibodies, polyclonal antibodies and immunology complexes, such as IP and Co-IP. The recombinant protein G ligand is coupled to 4% highly cross-linked agarose.

Specifications:
  • Resin: Cross-linked 4% agarose
  • Ligand: Recombinant Protein G
  • Shape: Sphere
  • Pore Size: 90 µm (45-165)
  • Support Density: 3 mg Protein G/ml wet gel
  • Binding Capacity: 20-25 mg h-IgG/ml wet gel
  • Maximum Flow Rate (25 °C): 300 cm/h
  • Recommended Flow Rate: < 150 cm/h
  • Highest Resisistance of Atmospheric Pressure: 0.3 MPa
  • pH Stability: 3-10
Affinity of Protein A/G for IgG types:
SourceIgG SubtypeAffinity for Protein AAffinity for Protein G
HumanIgG1++++++++
HumanIgG2++++++++
HumanIgG3-++++
HumanIgG4++++++++
MouseIgG1+++++
MouseIgG2a++++++++
MouseIgG2b++++++
MouseIgG3+++++
RabbitIgG+++++++
GoatIgG-++
HorseIgG++++++
DogIgG+++
CowIgG++++++
PigIgG++++++
MonkeyIgG++++++++


Target Protein G Resin
Storage Store at 2-8 °C (with 20% ethanol) for up to 2 years.
Buffer Note: Buffers are not included with this product.

Equilibration Buffer: 20 mM PBS, 150 mM KCl, pH 7.0
Elution Buffer: 20 mM citric acid, pH 3.0-4.0; or 100 mM glycine, pH 3.0; or 20 mM sodium acetate, pH 3.0-4.0
Neutralisation Buffer: 1 M Tris-HCl, pH 9.0
Availability Shipped within 10-20 working days.
Note This product is for research use only.
Directions for use Preparing the Protein G purification column:
  1. Thoroughly resuspend the protein G resin to achieve a homogeneous suspension of the resin in 20% ethanol storage buffer.
  2. Immediately transfer the resin into a purification column. Ensure that the bottom of the column is plugged with a stopper. Close the value of the column and allow the resin to settle.
  3. Equilibrate the column with 5-10 bed volume of equilibration buffer.
Preparing samples:

To avoid blocking the column, samples should be centrifuged and filtered through a 0.45 µm filter before loading.

Loading samples and washing:

Load samples and wash with 5-10 bed volume of equilibration buffer, and collect the flow-through in a tube

Elute:

Elute antibodies with elution buffer. Collect the elution containing the target immunoglobulin and immediately neutralise to pH > 7.0 with neutralisation buffer. The elution conditions are closely related with binding strength and stability of antibody. When necessary, optimise the elution buffer.

Regeneration of Protein G resin:
  1. Either:
    • Wash the column/resin with 3-5 bed volume of 0.1 M citric acid, or 0.1 M citric acid with 20% ethanol, and then 5 bed volume of PBS (pH 7.0); or
    • 3-5 bed volume of 0.05 M NaOH with 1 M NaCl, or 6 M GuHCl, and then 5 bed volume of deionised water.
  2. Store the column/resin in 20% ethanol.
Research Articles on Protein G Resin


Write a review

Tags:
(Click to show)