Proteinase K

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Catalogue No: abx098140
Price: US$72.50
(Size: 1 ml)

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Documents

Datasheet SDS
Proteinase K is a recombinant enzyme expressed in yeast. It is widely used for digestion of proteins in nucleic acid samples.

Target Proteinase K
Expression Recombinant
Tested Applications SDS-PAGE
Host Yeast
Recommended dilutions Molecular biology applications: 50-100 µg/ml. Optimal dilutions/concentrations should be determined by the end user.
Conjugation Unconjugated
Form Liquid
Purity ≥ 95% (SDS-PAGE)
Free from DNase and RNase.
Storage Store at -20 °C for up to 1 year. Avoid repeated freeze/thaw cycles.
Enzyme Commission Number 3.4.21.64
Molecular Weight 29 kDa
Buffer PBS, 10 mM Tris-HCl, pH 7.5, containing 5 mM CaCl2 and 50% glycerin.
CAS Number 39450-01-6
Biological Activity ≥ 30 U/mg
1 Unit (U) is defined as the amount of Proteinase K required to produce 1 µmol of Folin-positive amino acids using hemoglobin as the substrate, in 1 min at 37 °C.
Concentration 10 mg/ml
Availability Shipped within 5-10 working days.
Note THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
Directions for use Effective pH range: 4-12.5
Optimal pH range: 7.5-8.0
Optimal reaction temperature: 50-55 °C
Research Articles on Proteinase K


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