Proteinase K
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Catalogue No: abx082041
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US$522.00
(Size: 1 g)
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Datasheet SDS
Proteinase K is a recombinant enzyme expressed in yeast. It is widely used for digestion of proteins in nucleic acid samples.
Research Articles on Proteinase K
| Target | Proteinase K |
| Expression | Recombinant |
| Tested Applications | SDS-PAGE |
| Host | Yeast |
| Recommended dilutions | Molecular biology applications: 50-100 µg/ml. Optimal dilutions/concentrations should be determined by the end user. |
| Conjugation | Unconjugated |
| Form | Lyophilized |
| Biological Activity | ≥ 30 U/mg 1 Unit (U) is defined as the amount of Proteinase K required to produce 1 µmol of Folin-positive amino acids using hemoglobin as the substrate, in 1 min at 37 °C. |
| Purity | ≥ 95% (SDS-PAGE) Free from DNase and RNase. |
| Reconstitution | Reconstitute in a 50% glycerin solution. Ensure that the powder has fully dissolved before use. Use immediately after preparation, or store at 2-8 °C. For long-term storage, reconstitute to a concentration of 10-20 mg/ml and store at -20 °C. |
| Storage | Store between 2-8°C. |
| Shelf Life | 24 months. |
| Enzyme Commission Number | 3.4.21.64 |
| Molecular Weight | 29 kDa |
| Buffer | Prior to lyophilization: 10 mM Tris-HCl, pH 7.5, containing 5 mM CaCl2. |
| CAS Number | 39450-01-6 |
| Availability | Shipped within 5-10 working days. |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. |
| Directions for use | Effective pH range: 4-12.5 Optimal pH range: 7.5-8.0 Optimal reaction temperature: 50-55 °C |