26S Proteasome Non-ATPase Regulatory Subunit 2 (PSMD2) Cell ELISA Kit

Catalogue No: abx595823
Price: £375.00
(Size: 96 tests)
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PSMD2 Cell ELISA Kit is a cell-based ELISA Kit. Cells to be assayed should be seeded onto a clear flat bottom 96 well plate, using poly-L-lysine for non-adherent cells. Cells should be grown to 75-90% confluence and treated prior to carrying out the ELISA. The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the non-ATPase subunits of the 19S regulator lid. In addition to participation in proteasome function, this subunit may also participate in the TNF signalling pathway since it interacts with the tumor necrosis factor type 1 receptor. A pseudogene has been identified on chromosome 1. Alternative splicing results in multiple transcript variants of this gene.

Target 26S Proteasome Non-ATPase Regulatory Subunit 2 (PSMD2)
Reactivity Human, Mouse, Rat
Tested Applications ELISA
Recommended dilutions Optimal dilutions/concentrations should be determined by the end user.
Storage Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual.
Validity 6 months.
UniProt Primary AC Q13200 (UniProt, ExPASy)
UniProt Entry Name PSMD2_HUMAN
Gene Symbol PSMD2
GeneID 5708
OMIM 606223
NCBI Accession NP_001265637.1 NM_001278708.1 NP_001265638.1 NM_001278709.1 NP_002799.3 NM_002808.4
HGNC 9559
KEGG hsa:5708
Ensembl ENSG00000175166
String 9606.ENSP00000310129
Detection Method Colorimetric
Plate Coating Cell
Substrate TMB
Detection Antibody Conjugation HRP
Availability Shipped within 1-2 weeks.
Note This product is for research use only.

The range and sensitivity is subject to change. Please contact us for the latest product information. For accurate results, sample concentrations must be diluted to mid-range of the kit. If you require a specific range, please contact us in advance or write your request in your order comments.

Please note that our ELISA and CLIA kits are optimised for detection of native samples, rather than recombinant proteins. We are unable to guarantee detection of recombinant proteins, as they may have different sequences or tertiary structures to the native protein.
Research Articles on 26S Proteasome Non-ATPase Regulatory Subunit 2 (PSMD2)

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