qPCR Extraction Control Orange

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Catalogue No: abx461006
Price: US$6,293.00
(Size: 2000 rxns)

Orders to United States ship from United States

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qPCR Extraction Control Orange

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Download IFU SDS
qPCR Extraction Control Orange (emission wavelength 560 nm). This product consists of aritificial cells containing an internal control DNA sequence with no known homology to any organism, and a specific primer/probe control mix for PCR detection. It can be used to monitor the DNA extraction process in qPCR assays.

Target qPCR Extraction Control Orange
Tested Applications PCR
Form Liquid
Storage Store at -80°C. Avoid repeated freeze/thaw cycles.
Shelf Life Up to 12 months.
Buffer The exact formulation is proprietary.
Kit Components
Component Volume
Internal Control DNA 20 × 500 µl
25X Control Mix 20 × 100 µl
Availability Shipped within 10-20 working days.
Note THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.

This product is shipped with dry ice.
Directions for use All reactions should be carried out at room temperature unless stated otherwise.

Extraction Steps:
  1. Thaw and spin down all tubes before opening.
  2. Vortex the tube containing the Internal Control DNA to ensure complete mixing.
  3. Add 5 µl Internal Control DNA per sample to the lysis buffer (not provided). Ensure the mixture is well homogenised before loading onto samples (see next step).
  4. Follow the manufacturer's protocol for sample DNA extraction.
  5. The remaining Internal Control DNA mixture can be stored at 4 °C.
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Recommended reagent volumes per 25 µl qPCR mix:

Component Volume
2X PCR Master Mix (not provided) 12.5 µl
Target Probe/Primer Mix (not provided) N/A
Sample DNA from extraction step (not provided) N/A
Control Mix 1 µl
Total Volume 25 µl

Assay Setup:

Step Number of Cycles Temperature Time per Cycle
Polymerase Activation 1 cycle 95 °C 10 min
Denaturation30-40 cycles 95 °C 15 seconds
Annealing/Extension60 °C 30-60 seconds

Notes:
  • The fluorescence signal for the DNA Internal Control can be observed at 560 nm.
  • The qPCR conditions above are suitable for amplicons of up to 200 bp, however they can be varied to suit different qPCR mixes and machine-specific protocols.
  • Ct values of the Internal Control may vary due to elution volumes of nucleic acid and reagents used.
  • The optimal conditions will vary from reaction to reaction and are dependent on the system used.
  • Each parameter needs to be adjusted by the end user and some optimization may be required.
Research Articles on qPCR Extraction Control Orange


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