Rabbit and Mouse HRP/DAB Detection Kit

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Catalogue No: abx291501
Price: US$1,638.50
(Size: 50 ml)

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Datasheet SDS
Rabbit and Mouse HRP/DAB Detection Kit is a detection system containing DAB chromogen and a polymer-based secondary antibody conjugated to HRP, for use with rabbit IgG and/or mouse IgG primary antibodies in IHC.

Contents:

Size No. of tests Reagent A (Rabbit and Mouse HRP-conjugated Detection Reagent) Reagent B (Substrate Buffer) Reagent C (DAB Chromogen)
50 ml 250 50 ml 50 ml 2.5 ml
100 ml 500 10 ml 10 ml 5 ml


Target Rabbit and Mouse HRP/DAB Detection Kit
Tested Applications IHC
Recommended dilutions IHC: 0.2 ml/test. Optimal dilutions/concentrations should be determined by the end user.
Form Liquid
Storage Store in the dark at 2-8°C.
Buffer Reagent A: Contains stabilizing proteins and preservative. The exact contents are proprietary.
Reagent B: Contains hydrogen peroxide and preservative.
Reagent C: Contains DAB Chromogen solution.
Availability Shipped within 5-10 working days.
Note THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
Directions for use
  1. Prepare the Working Substrate Solution by adding 1 ml of Reagent B (Substrate buffer) and 20 µl of Reagent C (DAB Chromogen). Mix thoroughly. After preparation, use immediately or store between 2-8 °C for up to 5 days.
  2. Incubate the tissue section with the primary antibody and carry out wash procedures as required.
  3. Apply Reagent A (HRP-conjugated secondary antibody) to the section for 25-30 minutes.
  4. Apply the Working Substrate Solution to the section for 2-3 minutes.
Research Articles on Rabbit and Mouse HRP/DAB Detection Kit


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