RAD17 Checkpoint Clamp Loader Component (RAD17) Antibody

RAD17 Antibody is a Rabbit Polyclonal antibody against RAD17. The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Multiple alternatively spliced transcript variants of this gene, which encode four distinct protein isoforms, have been reported. Two pseudogenes, located on chromosomes 7 and 13, have been identified.

Target	RAD17 Checkpoint Clamp Loader Component (RAD17)
Clonality	Polyclonal
Reactivity	Human
Tested Applications	WB, IP
Host	Rabbit
Recommended dilutions	WB: 1/500 - 1/2000, IP: 0.5-4 µg/200-400 µg whole cell extracts. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 391-670 of human RAD17.
Isotype	IgG
Form	Liquid
Purification	Purified by affinity chromatography.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	O75943 (UniProt, ExPASy)
UniProt Entry Name	RAD17_HUMAN
Gene Symbol	RAD17
GeneID	5884
NCBI Accession	NP_002864.1
KEGG	hsa:5884
String	9606.ENSP00000426191
Molecular Weight	Calculated MW: 77 kDa Observed MW: 77 kDa
Buffer	PBS, pH 7.3, containing 0.02% sodium azide, 50% glycerol.
Concentration	> 0.2 mg/ml
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on RAD17 Checkpoint Clamp Loader Component (RAD17)

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