RAD9 (pS272) Antibody

Rad9 is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein is found to possess 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade.

Target	RAD9 (pS272)
Clonality	Polyclonal
Target Modification	Ser272
Modification	Phosphorylation
Reactivity	Human
Tested Applications	ELISA, WB, IHC
Host	Rabbit
Recommended dilutions	WB: 1/1000, IHC-P: 1/10 - 1/100. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	KLH-conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S272 of human RAD9.
Isotype	IgG
Form	Liquid
Purification	Purified by protein G affinity chromatography. Then, the antibody fraction was peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody was eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	Q99638 (UniProt, ExPASy)
UniProt Secondary AC	B2RCZ8, Q6FI29, Q96C41
NCBI Accession	NP_001230153.1 NP_004575.1
Molecular Weight	Calculated MW: 42.5 kDa
Buffer	PBS containing 0.09% sodium azide.
Specificity	Predicted to react with Monkey RAD9A.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on RAD9 (pS272)

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