Raf Proto-Oncogene Serine/Threonine Protein Kinase (RAF1) Antibody

Raf Proto-Oncogene Serine/Threonine Protein Kinase (RAF1) Antibody is a Rabbit Polyclonal antibody for the detection of RAF1.

Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2/cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. Regulates Rho signaling and migration, and is required for normal wound healing. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin (OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation.

Target	Raf Proto-Oncogene Serine/Threonine Protein Kinase (RAF1)
Clonality	Polyclonal
Reactivity	Human, Rat
Tested Applications	ELISA, WB, IHC
Host	Rabbit
Recommended dilutions	WB: 1/500 - 1/2000, IHC: 1/20 - 1/200. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	v-raf-1 murine leukemia viral oncogene homolog 1
Isotype	IgG
Form	Liquid
Purity	≥ 95% (SDS-PAGE)
Purification	Purified by immunogen affinity chromatography.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
Validity	12 months.
UniProt Primary AC	P04049 (UniProt, ExPASy)
UniProt Secondary AC	B0LPH8, B2R5N3, Q15278, Q9UC20
UniProt Entry Name	RAF1_HUMAN
Gene Symbol	RAF1
GeneID	5894
OMIM	164760
HGNC	9829
KEGG	hsa:5894
Ensembl	ENSG00000132155
String	9606.ENSP00000251849
Molecular Weight	Observed MW: 76 kDa
Buffer	PBS, pH 7.3, with 0.02% sodium azide and 50% glycerol.
Concentration	2 mg/ml
Availability	Shipped within 5-12 working days.
Note	This product is for research use only.

Research Articles on Raf Proto-Oncogene Serine/Threonine Protein Kinase (RAF1)

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