Raf Proto-Oncogene Serine/Threonine Protein Kinase (RAF1) Antibody

This gene is the cellular homolog of viral raf gene (v-raf).The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly.Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2.Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration.Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.

Target	Raf Proto-Oncogene Serine/Threonine Protein Kinase (RAF1)
Clonality	Monoclonal
Reactivity	Human
Tested Applications	ELISA, WB, IHC, FCM
Host	Mouse
Recommended dilutions	ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, FCM: 1/200 - 1/400. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	Purified recombinant fragment of human RAF1 expressed in E. coli.
Isotype	IgG1
Form	Liquid
Purification	Purified from ascites by Protein G chromatography.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	P04049 (UniProt, ExPASy)
UniProt Secondary AC	B0LPH8, B2R5N3, Q15278, Q9UC20
UniProt Entry Name	RAF1_HUMAN
Gene Symbol	RAF1
GeneID	5894
OMIM	164760
HGNC	9829
KEGG	hsa:5894
Ensembl	ENSG00000132155
String	9606.ENSP00000251849
Molecular Weight	73 kDa
Buffer	PBS, containing 0.05% sodium azide.
Concentration	1 mg/ml
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on Raf Proto-Oncogene Serine/Threonine Protein Kinase (RAF1)

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