RAF1 (pS296) Antibody
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Catalogue No: abx031933
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US$326.25
(Size: 80 µl)
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Datasheet SDS
Raf-1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration.
Research Articles on RAF1 (pS296)
| Target | RAF1 (pS296) |
| Clonality | Polyclonal |
| Target Modification | Ser296 |
| Modification | Phosphorylation |
| Reactivity | Human |
| Tested Applications | ELISA, DB |
| Host | Rabbit |
| Recommended dilutions | DB: 1/500. Optimal dilutions/concentrations should be determined by the end user. |
| Conjugation | Unconjugated |
| Immunogen | KLH-conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S296 of human RAF1. |
| Isotype | IgG |
| Form | Liquid |
| Purification | Purified by protein A affinity chromatography. Then, the antibody fraction was peptide affinity purified in a 2-step procedure with peptides. The antibody was eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
| Storage | Aliquot and store at 2-8°C for up to 2 weeks, or at -20°C for long term storage. Avoid repeated freeze/thaw cycles. |
| Shelf Life | 12 months. |
| UniProt Primary AC | P04049 (UniProt, ExPASy) |
| UniProt Secondary AC | B0LPH8, B2R5N3, Q15278, Q9UC20 |
| UniProt Entry Name | RAF1_HUMAN |
| KEGG | hsa:5894 |
| String | 9606.ENSP00000251849 |
| Molecular Weight | Calculated MW: 73.1 kDa |
| Buffer | PBS containing 0.09% sodium azide. |
| Specificity | Predicted to react with Mouse, Rat and Cow RAF1. |
| Availability | Shipped within 5-10 working days. |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. |