RAF1 (pS621) Antibody

RAF1 (pS621) Antibody is a Rabbit Polyclonal antibody against RAF1 (pS621). This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.

Target	RAF1 (pS621)
Clonality	Polyclonal
Reactivity	Human
Tested Applications	WB, IP
Host	Rabbit
Recommended dilutions	WB: 1/500 - 1/2000, IP: 0.5-4 µg/200-400 µg whole cell extracts. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	A synthetic phosphorylated peptide around S621 of human Phospho-Raf1-S621.
Isotype	IgG
Form	Liquid
Purification	Purified by affinity chromatography.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	P04049 (UniProt, ExPASy)
UniProt Secondary AC	B0LPH8, B2R5N3, Q15278, Q9UC20
UniProt Entry Name	RAF1_HUMAN
Gene Symbol	RAF1
GeneID	5894
NCBI Accession	NP_002871.1
KEGG	hsa:5894
String	9606.ENSP00000251849
Molecular Weight	Calculated MW: 73 kDa Observed MW: 73 kDa
Buffer	PBS, pH 7.3, containing 0.02% sodium azide, 50% glycerol.
Concentration	> 0.2 mg/ml
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on RAF1 (pS621)

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