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Rat Cathepsin G (CTSG) Protein (Active)
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Catalogue No: abx655977
Price:
US$536.50
(Size: 50 µg)
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Rat Cathepsin G (CTSG) is a recombinant active Rat protein produced in a Prokaryotic expression system (E. coli).
Research Articles on Cathepsin G (CTSG)
| Target | Cathepsin G (CTSG) |
| Origin | Rat |
| Expression | Recombinant |
| Tested Applications | WB, SDS-PAGE |
| Host | E. coli |
| Conjugation | Unconjugated |
| Form | Lyophilized |
| Activity | Active |
| Biological Activity | The activity was measured by the ability to cleave the peptide substrate Suc-Ala-Ala Pro-Phe-pNA. The reaction was performed in 160 mM Tris-HCl, 1.6 M NaCl, pH 7.4. Recombinant Rat CTSG was diluted to different concentrations and the reaction was initiated by adding 50 µl of CTSG to 50 µl of substrate (1 mM) and then read at 405 nm in kinetic mode for 5 minutes. The specific activity of recombinant rat CTSG is > 70 pmol/min/µg. |
| Purity | > 95% |
| Reconstitution | To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 10 mM PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in 10 mM PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex. |
| Storage | Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles. |
| UniProt Primary AC | G3V9Q7 (UniProt, ExPASy) |
| Molecular Weight | Calculated MW: 23.6 kDa Observed MW (SDS-PAGE): 24 kDa |
| Sequence Fragment | Gly51-Tyr250 |
| Sequence | GFLVREDFVL TAGHCFGSSI NVTLGAHNIR RQEGTQQHIT VLRAIRHPDY NPPPVIQNDI MLLQLRSRAR RSRAVKPVAL PQATKRVQPG ALCTVAGWGL VSQRRGTNVL QEVKLRVQTD QTCANRFQFY NSQTQICVGN PRERKSAFKG DSGGPLVCNN VAQGIVSYGS SSGNPPAVFT RIQSFMPWIK RTMRRLSSRY |
| Tag | N-terminal His tag |
| Buffer | Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 5% Trehalose. |
| Endotoxin Level | < 1.0 EU/µg (LAL method) |
| Concentration | Prior to lyophilization: 200 µg/ml |
| Availability | Shipped within 5-7 working days. |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. |