Rb (pS780) Antibody

RB1 likely acts as a regulator of other genes. It forms a complex with adenovirus E1A and with SV40 large T antigen, acts as a tumor suppressor, and may bind and modulate functionally certain cellular proteins with which T and E1A compete for pocket binding. RB1 is a potent inhibitor of E2F-mediated trans-activation, and also recruits and targets histone methyltransferase SUV39H1 leading to epigenetic transcriptional repression. This protein inhibits the intrinsic kinase activity of TAF1. Defects in RB1 are the cause of childhood cancer retinoblastoma (RB), a congenital malignant tumor that arises from the nuclear layers of the retina. Defects in RB1 are also a cause of bladder cancer and osteogenic sarcoma.

Target	Rb (pS780)
Clonality	Polyclonal
Target Modification	Ser780
Modification	Phosphorylation
Reactivity	Human
Tested Applications	ELISA, IHC, DB
Host	Rabbit
Recommended dilutions	IHC-P: 1/50 - 1/100, DB: 1/500. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	KLH-conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S780 of human Rb.
Isotype	IgG
Form	Liquid
Purification	Purified by protein G affinity chromatography. Then, the antibody fraction was peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody was eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	P06400 (UniProt, ExPASy)
UniProt Secondary AC	A8K5E3, P78499, Q5VW46, Q8IZL4
UniProt Entry Name	RB_HUMAN
KEGG	hsa:5925
String	9606.ENSP00000267163
Molecular Weight	Calculated MW: 106 kDa
Buffer	PBS containing 0.09% sodium azide.
Specificity	Predicted to react with Mouse and Rat RB1.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on Rb (pS780)

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