Replication Factor C Subunit 4 (RFC4) Antibody

RFC4 Antibody is a Rabbit Polyclonal antibody against RFC4. The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported.

Target	Replication Factor C Subunit 4 (RFC4)
Clonality	Polyclonal
Reactivity	Human, Rat
Tested Applications	WB, IF/ICC, IP
Host	Rabbit
Recommended dilutions	WB: 1/500 - 1/2000, IF/ICC: 1/50 - 1/100, IP: 0.5-4 µg/200-400 µg whole cell extracts. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 204-363 of human RFC4.
Isotype	IgG
Form	Liquid
Purification	Purified by affinity chromatography.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	P35249 (UniProt, ExPASy)
UniProt Secondary AC	B4DM41, D3DNV2, Q6FHX7
UniProt Entry Name	RFC4_HUMAN
Gene Symbol	RFC4
GeneID	5984
NCBI Accession	NP_002907.1
KEGG	hsa:5984
String	9606.ENSP00000376272
Molecular Weight	Calculated MW: 40 kDa Observed MW: 40 kDa
Buffer	PBS, pH 7.3, containing 0.02% sodium azide, 50% glycerol.
Concentration	> 0.2 mg/ml
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on Replication Factor C Subunit 4 (RFC4)

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